GAPDH anticorps (Internal Region)

N° du produit ABIN185543

L’anticorps Chèvre Polyclonal anti-GAPDH a été validé pour WB, ELISA, IHC et IF. Il convient pour détecter GAPDH dans des échantillons de Humain, Souris et Rat. Il y a 6+ publications disponibles.

Aperçu rapide pour GAPDH anticorps (Internal Region) (ABIN185543)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-GAPDH anticorps

Épitope: Internal Region

Fonction: GAPDH (Internal)

Séquence: GVNHEKYDNS LK

Specificité: This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GVNHEKYDNSLK, from the internal region of the protein sequence according to NP_002037.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1 kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-GAPDH (ABIN185543)

Ramsden, Nommiste, R Lane, Carr, Powner, J K Smart, Chen, Muthiah, Webster, Moore, Cheetham, da Cruz, Coffey: "Rescue of the MERTK phagocytic defect in a human iPSC disease model using translational read-through inducing drugs." dans: Scientific reports, Vol. 7, Issue 1, pp. 51, (2019) (PubMed).

Carr, Vugler, Yu, Semo, Coffey, Moss, Greenwood: "The expression of retinal cell markers in human retinal pigment epithelial cells and their augmentation by the synthetic retinoid fenretinide." dans: Molecular vision, Vol. 17, pp. 1701-15, (2011) (PubMed).

Carr, Vugler, Lawrence, Chen, Ahmado, Chen, Semo, Gias, da Cruz, Moore, Walsh, Coffey: "Molecular characterization and functional analysis of phagocytosis by human embryonic stem cell-derived RPE cells using a novel human retinal assay." dans: Molecular vision, Vol. 15, pp. 283-95, (2009) (PubMed).

Chakir, Daya, Aiba, Tunin, Dimaano, Abraham, Jaques-Robinson, Jacques, Lai, Pacak, Zhu, Xiao, Tomaselli, Kass: "Mechanisms of enhanced beta-adrenergic reserve from cardiac resynchronization therapy." dans: Circulation, Vol. 119, Issue 9, pp. 1231-40, (2009) (PubMed).

Schillace, Miller, Pisenti, Grotzke, Swarbrick, Lewinsohn, Carr: "A-kinase anchoring in dendritic cells is required for antigen presentation." dans: PLoS ONE, Vol. 4, Issue 3, pp. e4807, (2009) (PubMed).

Vugler, Carr, Lawrence, Chen, Burrell, Wright, Lundh, Semo, Ahmado, Gias, da Cruz, Moore, Andrews, Walsh, Coffey: "Elucidating the phenomenon of HESC-derived RPE: anatomy of cell genesis, expansion and retinal transplantation." dans: Experimental neurology, Vol. 214, Issue 2, pp. 347-61, (2008) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

ID gène: 2597, 14433, 24383

NCBI Accession: NP_002037, NP_001243728