IFT88 anticorps (C-Term)

N° du produit ABIN185555

L’anticorps Chèvre Polyclonal anti-IFT88 a été validé pour WB, IF, ELISA et FACS. Il convient pour détecter IFT88 dans des échantillons de Humain et Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour IFT88 anticorps (C-Term) (ABIN185555)

Antigène: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp IFT88 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-IFT88 anticorps

Épitope: C-Term

Fonction: IFT88 / Polaris

Séquence: KKRIDEDDFA DEE

Specificité: This antibody is expected to recognise many reported isoforms.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2, NP_006522.2.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 90 kDa band observed in lysates of cell line A549 and in Mouse Testis lysates, and in preliminary testing of Rat Testes lysate (calculated MW of 90.4 kDa according to Hunab NP_001340498.1, and 93.1 kDa according to Mouse NP_033402.2 93.

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nucleus and less staining in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-IFT88 (ABIN185555)

Miyoshi, Kasahara, Miyazaki, Shimizu, Taniguchi, Matsuzaki, Tohyama, Asanuma: "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 23, Issue 10, pp. 3289-97, (2009) (PubMed).

Jiang, Chiou, Wang, Chien, Ho, Tsai, Lin, Tsai, Li: "Essential role of nephrocystin in photoreceptor intraflagellar transport in mouse." dans: Human molecular genetics, Vol. 18, Issue 9, pp. 1566-77, (2009) (PubMed).

Détails sur IFT88

Antigène: IFT88 (Intraflagellar Transport 88 Homolog (IFT88))

Autre désignation: IFT88

Sujet: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar tra

ID gène: 8100, 21821, 305918

NCBI Accession: NP_783195, NP_006522, NP_001340507, NP_001340498

Pathways: Signalisation Hedgehog, Sensory Perception of Sound