LEF1 anticorps (Internal Region)

N° du produit ABIN185607

Cet anticorps anti-LEF1 est un anticorps Chèvre Polyclonal détectant LEF1 dans WB, ELISA, IF et FACS. Adapté pour Humain.

Aperçu rapide pour LEF1 anticorps (Internal Region) (ABIN185607)

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp LEF1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-LEF1 anticorps

Épitope: Internal Region

Fonction: LEF1

Séquence: QHEQRKEQEP KRPH

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-QHEQRKEQEPKRPH, from the internal region of the protein sequence according to NP_057353.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 50--55 kDa band observed in nuclear lysates of cell line K562 (calculated MW of 44.2 kDa according to NP_057353.1)/ This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the imm

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur LEF1

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Autre désignation: LEF1

Sujet: LEF1, lymphoid enhancer-binding factor 1 , HGNC:6551, DKFZp586H0919, TCF1ALPHA , lymphoid enhancer binding factor-1

ID gène: 51176, 16842, 161452

NCBI Accession: NP_057353

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding