Choline Acetyltransferase anticorps (C-Term)

N° du produit ABIN185635

Cet anticorps Chèvre Polyclonal détecte spécifiquement Choline Acetyltransferase dans WB, IHC et ELISA. Il présente une réactivité envers Souris et Rat.

Aperçu rapide pour Choline Acetyltransferase anticorps (C-Term) (ABIN185635)

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivité: Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Choline Acetyltransferase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-Choline Acetyltransferase anticorps

Épitope: C-Term

Fonction: Chat (mouse)

Séquence: KERARGPTKP SNLD

Specificité: This antibody is NOT expected to cross-react to Human CHAT.

Réactivité croisée: Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-KERARGPTKPSNLD, from the C Terminus of the protein sequence according to NP_034021.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Frozen sections of Mouse Forebrain shows staining of neural cell bodies. Optimal dilution: 1:2000.

Western Blot: Approx 75 kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3 kDa according to Mouse NP_034021.1 and Rat NP_001164064.1). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: Chat

Sujet: Chat, choline acetyltransferase , B230380D24Rik

ID gène: 12647, 432359

NCBI Accession: NP_034021

Pathways: Skeletal Muscle Fiber Development