NET1 anticorps (Internal Region)

N° du produit ABIN185644

Cet anticorps Chèvre Polyclonal détecte spécifiquement NET1 dans ELISA et IHC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour NET1 anticorps (Internal Region) (ABIN185644)

Antigène: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp NET1 est non-conjugé

Application: ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-NET1 anticorps

Épitope: Internal Region

Fonction: NET1 / ARHGEF8 (Internal)

Séquence: DKLEYLDEKQ RDPR

Specificité: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-DKLEYLDEKQRDPR, from the internal region of the protein sequence according to NP_005854.2, NP_001040625.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80-90 kDa double band in Human Placenta lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur NET1

Antigène: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Autre désignation: NET1

Sujet: NET1, ARHGEF8, neuroepithelial cell transforming gene 1, NET1A, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, small GTP-binding prot

ID gène: 10276, 56349, 307098

NCBI Accession: NP_005854, NP_001040625

Pathways: Neurotrophin Signaling Pathway