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PPARG anticorps (AA 311-500)

Cet anticorps anti-PPARG est un anticorps Lapin Polyclonal détectant PPARG dans WB, IHC, IP et ICC. Adapté pour Boeuf (Vache). Ce Primary Antibody a été cité dans 1 publication.
N° du produit ABIN1860282

Aperçu rapide pour PPARG anticorps (AA 311-500) (ABIN1860282)

Antigène

Voir toutes PPARG Anticorps
PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivité

  • 166
  • 116
  • 102
  • 34
  • 33
  • 23
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  • 11
  • 6
  • 6
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  • 2
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  • 2
  • 2
  • 1
Boeuf (Vache)

Hôte

  • 158
  • 25
  • 6
  • 2
Lapin

Clonalité

  • 165
  • 27
Polyclonal

Conjugué

  • 91
  • 15
  • 11
  • 6
  • 5
  • 4
  • 4
  • 4
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  • 2
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Cet anticorp PPARG est non-conjugé

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • Épitope

    • 26
    • 17
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    • 12
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    • 5
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    • 1
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    • 1
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    • 1
    • 1
    • 1
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    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    AA 311-500

    Fonction

    Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)

    Specificité

    The antibody is a rabbit polyclonal antibody raised against PPARg. It has been selected for its ability to recognize PPARg in immunohistochemical staining and western blotting.

     Réactivité croisée

    Humain, Souris, Rat

    Purification

    Protein A + Protein G affinity chromatography

    Immunogène

    Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg)

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    0.5 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze-thaw cycles.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    24 months
  • Montanari, Colitti: "Simpson-Golabi-Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation." dans: Histochemistry and cell biology, Vol. 149, Issue 6, pp. 593-605, (2018) (PubMed).

  • Antigène

    PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

    Autre désignation

    PPARg

    Sujet

    PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3

    Pathways

    Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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