PPARG anticorps (AA 280-475) (APC)
Aperçu rapide pour PPARG anticorps (AA 280-475) (APC) (ABIN1860285)
Antigène
Voir toutes PPARG AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 280-475
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Fonction
- Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
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Séquence
- MGHHHHHHSG SEF- F QGCQFRSVEA VQEITEYAKN IPGFVSLDLN DQVTLLKYGV HEIIYTMLAS LMNKDGVLIS EGQGFMTREF LKSLRKPFGD FMEPKFEFAV KFNALELDDS DLAIFIAVII LSGDRPGLLN VKPIEDIQDN LLQALELQLK LNHPEASQLF AKLLQKMTDL RQIVTEHVQL LQVIKKTETD MSLHPLLQEI YKDLY
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Specificité
- The antibody is a cavia polyclonal antibody raised against PPARg. It has been selected for its ability to recognize PPARg in immunohistochemical staining and western blotting.
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Réactivité croisée
- Souris
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Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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Immunogène
- RPA886Rb01Recombinant Peroxisome Proliferator Activated Receptor Gamma (PPARg)
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Isotype
- IgG
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Indications d'application
- Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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Commentaires
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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Agent conservateur
- ProClin
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Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Conseil sur la manipulation
- Avoid repeated freeze/thaw cycles
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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Date de péremption
- 12 months
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- PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))
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Autre désignation
- PPARg
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Sujet
- PPAR-G, PPARG1, PPARG2, NR1C3, Glitazone Receptor, Nuclear Receptor Subfamily 1 Group C Member 3
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Pathways
- Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Antigène
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