Pleiotrophin anticorps (AA 33-168)

N° du produit ABIN1860363

Détail du produit anti-Pleiotrophin anticorps

Antigène: Pleiotrophin (PTN)

Épitope: AA 33-168

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Pleiotrophin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Séquence: MGHHHHHHSG SEF- GKKEKPEK KVKKSDCGEW QWSVCVPTSG DCGLGTREGT RTGAECKQTM KTQRCKIPCN WKKQFGAECK YQFQAWGECD LNTALKTRTG SLKRALHNAD CQKTVTISKP CGKLTKPKPQ AESKKKKKEG KKQEKMLD

Specificité: It has been selected for its ability to recognize PTN in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant PTN expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against PTN.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant PTN (Gly33~Asp168) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur Pleiotrophin

Antigène: Pleiotrophin (PTN)

Autre désignation: Pleiotrophin (PTN Produits)

Synonymes: anticorps zHBNF, anticorps zgc:111787, anticorps harp, anticorps hbnf, anticorps X-PTF, anticorps hbgf8, anticorps negf1, anticorps ptn, anticorps PTN, anticorps HARP, anticorps HBGF8, anticorps HBNF, anticorps NEGF1, anticorps PTN-A, anticorps HB-GAM, anticorps HBBM, anticorps HBGF-8, anticorps OSF-1, anticorps HBBN, anticorps OSF, anticorps Osf-1, anticorps Osf1, anticorps Hbnf, anticorps pleiotrophin, anticorps pleiotrophin L homeolog, anticorps ptn, anticorps PTN, anticorps ptn.L, anticorps Ptn

Pathways: Signalisation RTK