RPA1 anticorps

N° du produit ABIN190714

Cet anticorps Lapin Polyclonal détecte spécifiquement RPA1 dans WB, IP et ChIP. Il présente une réactivité envers Saccharomyces cerevisiae et a été mentionné dans 2+ publications.

Aperçu rapide pour RPA1 anticorps (ABIN190714)

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RPA1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-RPA1 anticorps

Réactivité croisée (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Homologie: Saccharomyces cerevisiae

Attributs du produit: Expected / apparent Molecular Weight of the Antigene: 70 + 30 + 14 kDa

Purification: serum

Immunogène: RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa), overexpressed in E.coli and purified by chromatography, no affinity tags were added to any of three subunits

Information d'application

Indications d'application: 1: 20 000 with standard ECL (WB), chromatin immunoprecipiation

Commentaires:

antibody was also successfully used in ChIP application, presented data are courtesy of M. Pool and Dr. H. van Attikum

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Conseil sur la manipulation: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Références pour anticorps anti-RPA1 (ABIN190714)

Chen, Lisby, Symington: "RPA coordinates DNA end resection and prevents formation of DNA hairpins." dans: Molecular cell, Vol. 50, Issue 4, pp. 589-600, (2013) (PubMed).

Bentsen, Nielsen, Lisby, Nielsen, Gupta, Mundbjerg, Andersen, Bjergbaek: "MRX protects fork integrity at protein-DNA barriers, and its absence causes checkpoint activation dependent on chromatin context." dans: Nucleic acids research, Vol. 41, Issue 5, pp. 3173-89, (2013) (PubMed).

Détails sur RPA1

Antigène: RPA1 (Replication Protein A1, 70kDa (RPA1))

Autre désignation: RFA

Sujet: Saccharomyces cerevisiaereplication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody

Poids moléculaire: 70 + 30 + 14 kDa

UniProt: P22336, P26754

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA