FH anticorps (Internal Region)

N° du produit ABIN190758

Cet anticorps anti-FH est un anticorps Chèvre Polyclonal détectant FH dans WB, ELISA, IHC et EIA. Adapté pour Humain.

Aperçu rapide pour FH anticorps (Internal Region) (ABIN190758)

Antigène: FH (Fumarate Hydratase (FH))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FH est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Classe de qualité: Verified

Détail du produit anti-FH anticorps

Épitope: Internal Region

Fonction: Fumarase / FH

Séquence: HPNDHVNKSQ SSND

Homologie: Human, Mouse, Rat, Dog

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-HPNDHVNKSQSSND, from the internal region of the protein sequence according to NP_000134.2.

Isotype: IgG

Information d'application

Indications d'application:

DS WB Results: Approx 48 kDa band observed in Human Kidney, Liver and Lung lysates (calculated MW of 54.6 kDa according to NP_000134.2). The observed molecular weight corresponds to earlier findings with antibodies from different sources. In transfected HEK293 transiently expressing Fumarase a band of approx. 55 kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.01-0.03 μg/mL.

DS IHC Results: Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Fumarase captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur FH

Antigène: FH (Fumarate Hydratase (FH))

Autre désignation: Fumarate Hydratase

Sujet: Fumarate hydratase, HLRCC, LRCC, MCL, MCUL1, fumarase, leiomyomatosis and renal cell cancer, multiple hereditary cutaneous leiomyomata

ID gène: 2271

NCBI Accession: NP_000134