Glucose-6-Phosphate Dehydrogenase anticorps (AA 308-320)

N° du produit ABIN190766

L’anticorps Chèvre Polyclonal anti-Glucose-6-Phosphate Dehydrogenase a été validé pour WB, IHC et ELISA. Il convient pour détecter Glucose-6-Phosphate Dehydrogenase dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Glucose-6-Phosphate Dehydrogenase anticorps (AA 308-320) (ABIN190766)

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Glucose-6-Phosphate Dehydrogenase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Classe de qualité: Verified

Détail du produit anti-Glucose-6-Phosphate Dehydrogenase anticorps

Épitope: AA 308-320

Fonction: G6PD (aa 308 - 320)

Séquence: STNSDDVRDE KVK

Specificité: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 55-60 kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5 kDa according to NP_000393.4 and 59.3 kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-Glucose-6-Phosphate Dehydrogenase (ABIN190766)

Hwang, Mruk, Rahighi, Raub, Chen, Dorn, Horikoshi, Wakatsuki, Chen, Mochly-Rosen: "Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator." dans: Nature communications, Vol. 9, Issue 1, pp. 4045, (2019) (PubMed).

Détails sur Glucose-6-Phosphate Dehydrogenase

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Autre désignation: G6PD

Sujet: G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PD

ID gène: 2539, 14381, 24377

NCBI Accession: NP_000393, NP_001035810

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones