ABHD5 anticorps (Internal Region)

N° du produit ABIN190850

L’anticorps Chèvre Polyclonal anti-ABHD5 a été validé pour WB, ELISA, IHC et IF. Il convient pour détecter ABHD5 dans des échantillons de Humain et Souris. Il y a 1 publication disponible.

Aperçu rapide pour ABHD5 anticorps (Internal Region) (ABIN190850)

Antigène: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ABHD5 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-ABHD5 anticorps

Épitope: Internal Region

Fonction: CGI58 / ABHD5

Séquence: FPERPDLADQ DR

Specificité: This antibody is not expected to cross-react with ABHD4.

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence according to NP_057090.2.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Pancreas and Kidney. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell lines NIH3T3/3TL (calculated MW of 39.1 kDa according to NP_057090.2). Additional faint bands of 26 kDa and 18 kDa were also observed, and were successfully blocked by incubation with the immunizin

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm/ vesicles of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Références pour anticorps anti-ABHD5 (ABIN190850)

Koltes, Spurlock: "Coordination of lipid droplet-associated proteins during the transition period of Holstein dairy cows." dans: Journal of dairy science, Vol. 94, Issue 4, pp. 1839-48, (2011) (PubMed).

Détails sur ABHD5

Antigène: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Autre désignation: ABHD5

Sujet: ABHD5, CGI58 protein, aCGI58, bhydrolase domain containing 5, CDS, IECN2, MGC8731, NCIE2

ID gène: 51099, 67469, 316122

NCBI Accession: NP_057090

Pathways: Lipid Metabolism