CD31 anticorps (PE)

N° du produit ABIN192183

Cet anticorps anti-CD31 est un anticorps Souris Monoclonal détectant CD31 dans FACS. Adapté pour Humain. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour CD31 anticorps (PE) (ABIN192183)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est conjugé à/à la PE

Application: Flow Cytometry (FACS)

Clone: MEM-05

Détail du produit anti-CD31 anticorps (PE)

Fonction: Anti-Hu CD31 PE

Specificité: The antibody MEM-05 reacts with an extracellular epitope of CD31 (PECAM-1), a 130-140 kDa type I transmembrane glycoprotein expressed on monocytes, platelets, granulocytes, endothelial cells and stem cells of the myeloid lineage.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Leukocytes of a patient suffering from LGL-type leukaemia

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD31 (PE) (ABIN192183)

Kalina, Bakardjieva, Blom, Perez-Andres, Barendregt, Kanderová, Bonroy, Philippé, Blanco, Pico-Knijnenburg, Paping, Wolska-Kuśnierz, Pac, Tkazcyk, Haerynck, Akar, Formánková, Freiberger, Svatoň et al.: "EuroFlow Standardized Approach to Diagnostic Immunopheneotyping of Severe PID in Newborns and Young Children. ..." dans: Frontiers in immunology, Vol. 11, pp. 371, (2020) (PubMed).

van Dongen, van der Burg, Kalina, Perez-Andres, Mejstrikova, Vlkova, Lopez-Granados, Wentink, Kienzler, Philippé, Sousa, van Zelm, Blanco, Orfao: "EuroFlow-Based Flowcytometric Diagnostic Screening and Classification of Primary Immunodeficiencies of the Lymphoid System." dans: Frontiers in immunology, Vol. 10, pp. 1271, (2019) (PubMed).

Cârţână, Săftoiu, Gruionu, Gheonea, Pirici, Georgescu, Ciocâlteu, Gruionu: "Confocal laser endomicroscopy for the morphometric evaluation of microvessels in human colorectal cancer using targeted anti-CD31 antibodies." dans: PLoS ONE, Vol. 7, Issue 12, pp. e52815, (2013) (PubMed).

Prager, Staffler, Majdic, Säemann, Godár, Zlabinger, Stockinger: "Induction of hyporesponsiveness and impaired T lymphocyte activation by the CD31 receptor:ligand pathway in T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 4, pp. 2364-71, (2001) (PubMed).

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31

Sujet: Platelet and endothelial cell adhesion molecule 1,CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.,PECAM-1, EndoCAM, GPIIA, PECA1

ID gène: 5175

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization