STIM1 anticorps (C-Term)

N° du produit ABIN192394

Détail du produit anti-STIM1 anticorps

Antigène: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Épitope: C-Term

Reactivité: Humain, Rat, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp STIM1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificité: The mouse monoclonal antibody CDN3H4 reacts with a cytoplasmic epitope of human and rodent STIM1, a 84 kDa essential and conserved regulator of store-operated Ca2+ channel function.

Réactivité croisée (Details): Human, Mouse, Rat

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Synthetized peptide (C-terminal cytoplasmic part of STIM1).

Clone: CDN3H4

Isotype: IgG1

Information d'application

Indications d'application: Immunocytochemistry: Methanol-aceton fixation, positive control: HeLa human cervix carcinoma cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
Western blotting: Recommended dilution: 1 μg/mL, positive control: RBL rat basophilic leukemia cell line, both reducing and non-reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-STIM1 (ABIN192394)

Hájková, Bugajev, Dráberová, Vinopal, Dráberová, Janá?ek, Dráber, Dráber: "STIM1-directed reorganization of microtubules in activated mast cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 2, pp. 913-23, (2011) (PubMed).

Ng, Ramduny, Airey, Singer, Keller, Shen, Tian, Valencik, Hume: "Orai1 interacts with STIM1 and mediates capacitative Ca2+ entry in mouse pulmonary arterial smooth muscle cells." dans: American journal of physiology. Cell physiology, Vol. 299, Issue 5, pp. C1079-90, (2010) (PubMed).

Détails sur STIM1

Antigène: STIM1 (Stromal Interaction Molecule 1 (STIM1))

Autre désignation: STIM1 (STIM1 Produits)

Synonymes: anticorps stim1, anticorps MGC76045, anticorps STIM1, anticorps si:dkey-24p1.5, anticorps MGC154748, anticorps si:ch211-112l17.2, anticorps Stim1, anticorps D11S4896E, anticorps GOK, anticorps TAM, anticorps SIM, anticorps stromal interaction molecule 1, anticorps stromal interaction molecule 1a, anticorps stromal interaction molecule 1 L homeolog, anticorps stromal interaction molecule 1b, anticorps STIM1, anticorps stim1, anticorps stim1a, anticorps stim1.L, anticorps stim1b, anticorps Stim1

Sujet: Stromal interaction molecule 1,STIM1 (stromal interacting molecule, also known as GOK) acts as a sensor of calcium depletion within the endoplasmic reticulum and transduces the signal to Orai1, the presumptive CRAC channel at the plasma membrane. Following decrease of luminal calcium concentration, STIM1 oligomerizes and induces Orai1 to enable entry of extracellular calcium into the cytoplasm. However, the precise mechanism of STIM1-Orai1 interaction has not been elucidated yet. Many questions also remain to be solved around STIM1 functional distribution. It turns out that STIM1 associates with growing ends of microtubules and is involved in endoplasmic reticulum tubule extension.,GOK, TAM1, STRMK

ID gène: 6786

UniProt: G0XQ39

Pathways: TCR Signaling, BCR Signaling