MAPT anticorps (pThr231)

N° du produit ABIN196806

Cet anticorps anti-MAPT est un anticorps Lapin Polyclonal détectant MAPT dans WB et IHC (p). Adapté pour Humain, Rat et Souris.

Aperçu rapide pour MAPT anticorps (pThr231) (ABIN196806)

Antigène: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-MAPT anticorps

Épitope: pThr231

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around the phosphorylation site of Threonine 231 (V-R-Tp-P-P) derived from Human Tau .

Information d'application

Indications d'application: Western blot: 1/500approx. 1/1000. Immunohistochemistry: 1/50approx. 1/100 (Suitable for Paraffin Embedded Sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur MAPT

Antigène: MAPT (Microtubule-Associated Protein tau (MAPT))

Autre désignation: MAPT / tau

Sujet: Tau is a neuronal microtubule associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerisation and stabilise microtubules, but it also serves to link certain signalling pathways to the cytoskeleton. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF) and neurofibrillary lesions in Alzheimer's disease (AD) brain. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilisation of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Hyperphosphorylated tau is also found in a range of other central nervous system disorders. Numerous serine/threonine kinases, including GSK3 beta, PKA, Cdk5, and casein kinase II can phosphorylate Tau.Synonyms: MAPTL, MTBT1, Microtubule-associated protein tau, Neurofibrillary tangle protein, PHF-tau, Paired helical filament-tau

ID gène: 9606

UniProt: P10636

Pathways: Signalisation MAPK, Dynamique des Microtubules, M Phase, Regulation of Cell Size