Retinoblastoma 1 anticorps (pSer807)
Aperçu rapide pour Retinoblastoma 1 anticorps (pSer807) (ABIN196840)
Antigène
Voir toutes Retinoblastoma 1 (RB1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- pSer807
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Specificité
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Rb (phospho-Ser807) antibody detects endogenous levels of Rb only when phosphorylated at serine 807.
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Purification
- Immunoaffinity chromatography.
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Immunogène
- The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 807 (Y-I-SP-P-L).
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Indications d'application
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Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- Retinoblastoma 1 (RB1)
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Autre désignation
- Retinoblastoma-Associated Protein / RB1
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Sujet
- Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
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ID gène
- 5925
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NCBI Accession
- NP_000312
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UniProt
- P06400
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Pathways
- Cycle Cellulaire, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
Antigène
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