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NCOA1 anticorps (pTyr529)

Cet anticorps Lapin Polyclonal détecte spécifiquement NCOA1 dans WB et IHC (p). Il présente une réactivité envers Humain.
N° du produit ABIN196876

Aperçu rapide pour NCOA1 anticorps (pTyr529) (ABIN196876)

Antigène

Voir toutes NCOA1 Anticorps
NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivité

  • 61
  • 38
  • 18
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Humain

Hôte

  • 67
  • 9
Lapin

Clonalité

  • 57
  • 19
Polyclonal

Conjugué

  • 43
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp NCOA1 est non-conjugé

Application

  • 55
  • 16
  • 13
  • 13
  • 11
  • 11
  • 7
  • 6
  • 4
  • 4
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • Épitope

    • 15
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr529

    Specificité

    The antibody detects endogenous levels of Src only when phosphorylated at Tyrosine 529.

    Purification

    Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

    Immunogène

    The antiserum was produced against synthesized phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
  • Indications d'application

    Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    -20 °C

    Stockage commentaire

    Store the antibody (in aliquots) at -20 °C.
  • Antigène

    NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

    Autre désignation

    SRC1

    Sujet

    Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

    ID gène

    6714

    NCBI Accession

    NP_005408

    UniProt

    P12931

    Pathways

    Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha
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