CAMK2A anticorps (pThr286)

N° du produit ABIN197054

L’anticorps Lapin Polyclonal anti-CAMK2A a été validé pour WB et IF. Il convient pour détecter CAMK2A dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CAMK2A anticorps (pThr286) (ABIN197054)

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CAMK2A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-CAMK2A anticorps

Épitope: pThr286

Specificité: This antibody detects endogenous levels of CaMKII only when phosphorylated at Threonine 286.

Purification: Affinity-Chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from Human CaMKII around the phosphorylation site of Threonine 286 (Q-E-Tp-V-D).

Information d'application

Indications d'application: Western Blot: 1/500approx. 1/1000. Incubate membrane with diluted antibody in 5 % nonfat milk, 1X TBS, 0.1 % Tween-20 at 4 °Cwith gentle shaking, overnight. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur CAMK2A

Antigène: CAMK2A (Calcium/calmodulin-Dependent Protein Kinase II alpha (CAMK2A))

Autre désignation: CAMK2A

Sujet: CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.Synonyms: CAMK2A, CAMK2B, CAMKA, CaM kinase II subunit alpha, CaM kinase II subunit beta, CaM-kinase II alpha chain, CaM-kinase II beta chain, CaMK-II subunit alpha, CaMK-II subunit beta, Calcium/calmodulin-dependent protein kinase type II alpha chain, KIAA0968

ID gène: 815

NCBI Accession: NP_741960

UniProt: Q9UQM7

Pathways: Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction