PKC mu anticorps (Ser738)

N° du produit ABIN197312

L’anticorps anti-PKC mu Polyclonal Lapin est utilisé pour la détection de PKC mu dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IF.

Aperçu rapide pour PKC mu anticorps (Ser738) (ABIN197312)

Antigène: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PKC mu est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-PKC mu anticorps

Épitope: Ser738

Specificité: This antibody detects endogenous levels of total PKD/PKCμ protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from human PKD/PKCmy around the phosphorylation site of serine 738 (E-K-Sp-F-R).

Information d'application

Indications d'application: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur PKC mu

Antigène: PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))

Autre désignation: PRKD1

Sujet: Protein Kinase C mu, is a ~140 kDa member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine, and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, metastasis and transcriptional regulation. PKC mu (also known as Protein Kinase D or PKD) is implicated in the regulation of multiple cellular processes including Golgi organization and membrane transport in epithelial cells. PKC mu is phosphorylated on serine 742 (serine 748 for the mouse sequence) in the activation loop in a PKC-dependent pathway, mainly by PKC eta and PKC epsilon. This is critical for its catalytic activity, substrate phosphorylation and role in activating the ERK1 MAP Kinase signaling cascade.Synonyms: PKC D1, PKC mu, PKD, PKD1, PRKCM, Protein kinase C mu type, Protein kinase D, Serine/threonine-protein kinase D1, nPKC-D1, nPKC-mu

ID gène: 5587

NCBI Accession: NP_002733

UniProt: Q15139

Pathways: Myometrial Relaxation and Contraction