HDAC4 anticorps (Ser632)

N° du produit ABIN197338

Cet anticorps Lapin Polyclonal détecte spécifiquement HDAC4 dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour HDAC4 anticorps (Ser632) (ABIN197338)

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-HDAC4 anticorps

Épitope: Ser632

Specificité: This antibody AP02668PU detects endogenous levels of total HDAC4 protein.

Purification: Immunoaffinity Chromatography using epitope-specific immunogen.

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from Human HDAC4 around the phosphorylation site of Serine 632 (A-Q-Sp-S-P).

Information d'application

Indications d'application: Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur HDAC4

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Autre désignation: HDAC4

Sujet: Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART.Synonyms: HD4, Histone deacetylase 4, KIAA0288

ID gène: 9759

NCBI Accession: NP_006028

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process