BIM anticorps (Ser65)

N° du produit ABIN197552

L’anticorps Lapin Polyclonal anti-BIM a été validé pour WB et IHC (p). Il convient pour détecter BIM dans des échantillons de Humain et Souris.

Aperçu rapide pour BIM anticorps (Ser65) (ABIN197552)

Antigène: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BIM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BIM anticorps

Épitope: Ser65

Specificité: BIM antibody detects endogenous levels of total BIM protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Immunogène: The antiserum was produced against synthesized non-phosphopeptide derived from Mouse BIM around the phosphorylation site of Serine 65 (P-A-Sp-P-G).

Information d'application

Indications d'application: Immunohistochemistry: 1/50approx. 1/100. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur BIM

Antigène: BIM (BCL2L11) (BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))

Sujet: Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw. Bim is sequestered in an inactive conformation through binding to the microtubule-associated dynein motor complex. Certain apoptotic stimuli release Bim from microtubules, allowing inhibitory binding to anti-apoptotic Bcl-2 family members and subsequent iniation of apoptosis.Synonyms: BCL2L11, BIM, Bcl2-L-11, Bcl2-interacting mediator of cell death, BimEL, BimL, BimS

ID gène: 10018

NCBI Accession: NP_001191035

UniProt: O43521

Pathways: Signalisation PI3K-Akt, Neurotrophin Signaling Pathway, Tube Formation, Positive Regulation of Endopeptidase Activity