MARCKS anticorps (Ser158)
Aperçu rapide pour MARCKS anticorps (Ser158) (ABIN197560)
Antigène
Voir toutes MARCKS AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- Ser158
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Specificité
- This antibody detects endogenous levels of total MARCKS protein.
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Purification
- Immunoaffinity Chromatography using epitope-specific immunogen.
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Immunogène
- The antiserum was produced against synthesized non-phosphopeptide derived from human MARCKS around the phosphorylation site of serine 158 (R-F-Sp-F-K).
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Indications d'application
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Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))
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Autre désignation
- MARCKS
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Sujet
- MARCKS, (Myristoylated Alanine-Rich C Kinase Substrate), is a member of a family of calmodulin binding proteins and is a major substrate for phosphorylation by protein kinase C (PKC). The phosphorylation of Ser152/156 can be used as a measure of PKC activation. Phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL
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ID gène
- 4082
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NCBI Accession
- NP_002347
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UniProt
- P29966
Antigène
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