Perforin 1 anticorps

N° du produit ABIN1981903

Cet anticorps Souris Monoclonal détecte spécifiquement Perforin 1 dans IP, FACS, ICC, IHC (fro) et IHC (p). Il présente une réactivité envers Humain et Boeuf (Vache) et a été mentionné dans 4+ publications.

Aperçu rapide pour Perforin 1 anticorps (ABIN1981903)

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivité: Humain, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Perforin 1 est non-conjugé

Application: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: DG9

Détail du produit anti-Perforin 1 anticorps

Fonction: Anti-Perforin Purified

Specificité: The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Human, Bovine

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: purified granules from human YT lymphoma cell line

Isotype: IgG2b kappa

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 3-12 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze. Do not use after expiration date stamped on vial label.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-Perforin 1 (ABIN1981903)

Li, Jeong, Song: "Molecular Characteristics and Distribution of Adult Human Corneal Immune Cell Types." dans: , Vol. 13, pp. 798346, (2022) (PubMed).

Qiu, Chen, Liao, Zhang, Wang, Li, Luo, Fang, Li, Zhou, Shen, Chen, Huang, Cai, Cao, Jiang, Zeng, Chen: "Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions." dans: PLoS pathogens, Vol. 8, Issue 11, pp. e1002984, (2012) (PubMed).

Takeuchi, Inoue, Otani, Yamasaki, Nakamura, Kibata: "Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT." dans: Journal of molecular cell biology, Vol. 2, Issue 3, pp. 139-51, (2010) (PubMed).

Hersperger, Makedonas, Betts: "Flow cytometric detection of perforin upregulation in human CD8 T cells." dans: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 73, Issue 11, pp. 1050-7, (2008) (PubMed).

Détails sur Perforin 1

Antigène: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Autre désignation: Perforin

Sujet: Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2

ID gène: 5551

UniProt: P14222

Pathways: Apoptose, Caspase Cascade in Apoptosis