GJA3 anticorps (Internal Region)

N° du produit ABIN200660

L’anticorps Lapin Polyclonal anti-GJA3 a été validé pour WB, IHC, IF et IHC (fro). Il convient pour détecter GJA3 dans des échantillons de Souris.

Aperçu rapide pour GJA3 anticorps (Internal Region) (ABIN200660)

Antigène: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GJA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-GJA3 anticorps

Épitope: Internal Region

Specificité: Recognizes mouse Connexin 46 protein. Species sequence homology: rat, Chinese hamster and Golden Hamster.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide derived from an internal region of the mouse Connexin 46 protein.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IF (3 μg/mL), IHC, IHC-Fr, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Immunohistochemistry (Frozen): 3 μg/mL. Western Blot: 1-3 μg/mL. Identifies a single band at ~46kD. Immunofluorescence: 3 μg/mL. Note: For IHC, mice were perfused transcardially with 3 mL pre-fixative (50 mM phosphate buffer, 0.1 % sodium nitrite, 1 unit/mL heparin), followed by 40 mL fixative (0.16M sodium phosphate buffer,  pH 7.6, 0.2 % picric acid, and 1-, 2-, or 4 % paraformaldehyde), and perfusion with 10 mL sucrose wash (10 % sucrose in 25 mM sodium phosphate buffer,  pH 7.4. Tissues were then stored in cryoprotectant (10 % sucrose, 25 mM phosphate buffer,  pH 7.4, 0.04 % sodium azide) for a minimum of 24 hours prior to sectioning.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GJA3

Antigène: GJA3 (Gap Junction Protein, alpha 3, 46kDa (GJA3))

Autre désignation: GJA3 / CX46 / Connexin 46

Sujet: Name/Gene ID: GJA3
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJA3, Connexin-46, CX46, CZP3, Gap junction alpha-3 protein, Gap junction protein alpha 3, CAE3, Connexin 46

ID gène: 2700

UniProt: Q9Y6H8