ITGAV/ITGB6 anticorps

N° du produit ABIN201125

L’anticorps anti-ITGAV/ITGB6 Monoclonal Souris est utilisé pour la détection de ITGAV/ITGB6 dans des échantillons de Humain, Souris et Rat. Il a été validé pour FACS, IP et Func.

Aperçu rapide pour ITGAV/ITGB6 anticorps (ABIN201125)

Antigène: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGAV/ITGB6 est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Détail du produit anti-ITGAV/ITGB6 anticorps

Specificité: Recognizes mouse integrin alpha V beta 6. Blocks activity against mouse and human alpha V beta 6. Does not recognize alpha V or other alpha V integrins. Species cross-reactivity: Human, rat and mouse. Because the antibody was raised in knockout mice, it is likely to recognize the receptor in other species.

Purification: Protein A purified

Immunogène: Immunization of beta 6 knockout mice with wild type murine keratinocytes.

Type of Immunogen: Cells

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, Func, IP

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation and Function Blocking. Not suitable for use in Western Blot and Immunohistochemistry. Flow Cytometry (effective for mouse and human): Use buffers containing 1 mM Mg, since the epitope recognized is divalent cation-dependent. Immunoprecipitation: Effective. Protein G or rabbit anti-mouse IgG recommended for capture. Function blocking: Inhibition of alpha V beta 6-mediated cell adhesion and cell migration are achieved at a concentration of 100 μg/mL using beta 6 transfected cells tested against TGFbeta LAP. Note: Cell lines adhere to simple ligands (e. g. fibronectin) using more integrins than alphav/beta6, so it would be necessary to also block other integrins (e. g. with anti-beta1 antibody) to see any effect of this antibody on adhesion to fibronectin. For example, H441 cells, which naturally express high levels of alphaV/beta6, may be used as a positive control.

Not recommended for: IHC, WB

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS. No preservatives added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ITGAV/ITGB6

Antigène: ITGAV/ITGB6 (Integrin alpha V, Integrin beta 6 (ITGAV/ITGB6))

Autre désignation: Integrin AlphaV Beta6