NOS1 anticorps (AA 724-739)

N° du produit ABIN201344

L’anticorps Lapin Polyclonal anti-NOS1 a été validé pour WB, IHC, ELISA et IF. Il convient pour détecter NOS1 dans des échantillons de Humain.

Aperçu rapide pour NOS1 anticorps (AA 724-739) (ABIN201344)

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NOS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Détail du produit anti-NOS1 anticorps

Épitope: AA 724-739

Specificité: Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

Purification: Affinity purified

Immunogène: Ved in human, rat and mouse. IMMUNOGEN: Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Information d'application

Indications d'application: Approved: ELISA, IF, IHC, WB (1 - 10 μg/mL)

Usage: Immunohistochemistry: Use at 5-10 μg/mL on rat neuronal sections fixed with 3.5 % paraformaldehyde. Incubation overnight at 4?C with a 1:100-1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons. Western blot: 1-10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155kD protein. ELISA: Use at 1.0 μg/mL or lower on plates coated with 50-100 ng of peptide antigen.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur NOS1

Antigène: NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))

Autre désignation: NOS1 / nNOS

Sujet: Name/Gene ID: NOS1

Synonyms: NOS1, BNOS, Constitutive NOS, IHPS1, NNOS, NC-NOS, Nitric oxide synthase, brain, N-NOS, Neuronal NOS, NOS, NOS type I, Nos, type i

ID gène: 4842

UniProt: P29475

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction