Topoisomerase II alpha anticorps (C-Term)

N° du produit ABIN201464

L’anticorps Souris Monoclonal anti-Topoisomerase II alpha a été validé pour WB, IHC, FACS, IHC (p), IP et ICC. Il convient pour détecter Topoisomerase II alpha dans des échantillons de Humain.

Aperçu rapide pour Topoisomerase II alpha anticorps (C-Term) (ABIN201464)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: C-Term

Specificité: Recognizes a major protein of 170kD which was identified as the human alpha isoform of topoisomerase II. Recognizes a carboxy terminal alpha-isoenzyme specific epitope missing in topoisomerase II beta.

Purification: Purified

Immunogène: Nuclear protein preparation from the human cell line U937.50.

Type of Immunogen: Cells

Isotype: IgG2a

Information d'application

Indications d'application: Approved: Flo, ICC (5 - 10 μg/mL), IHC, IHC-P, IP, WB (1 - 10 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Western Blot: 1-10 μg/mL. Immunohistochemistry: 5-10 μg/mL. Strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections. Immunocytochemistry: 5-10 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: TOP2A / Topoisomerase II alpha

Sujet: Name/Gene ID: TOP2A
Family: Topoisomerase

Synonyms: TOP2A, DNA topoisomerase II, 170 kD, TOP2, DNA gyrase, DNA topoisomerase 2-alpha, TP2A

ID gène: 7153

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases