Complement C4d anticorps

N° du produit ABIN201862

Cet anticorps Souris Monoclonal détecte spécifiquement Complement C4d dans IF, ELISA, IHC, WB, IHC (p) et IHC (fro). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Complement C4d anticorps (ABIN201862)

Antigène: Complement C4d (C4d) (Complement Component C4d (C4d))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Complement C4d est non-conjugé

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 10-11

Détail du produit anti-Complement C4d anticorps

Specificité: Recognizes the secreted protein complement component 4d (C4d). The presence of C4d in renal peritubular capillaries is a key indicator for acute antibody-mediated rejection [AMR]. C4d was accepted in 2003 into the Banff classification for identification of acute AMR. Is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection. Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4ba€™s alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ba€™s short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. Is used to detect the biomarker C4d which has been described as a a€oefootprinta€ of antibody mediated tissue rejection. The internal thioester of C4b becomes exposed during cleavage to C4d and forms a covalent bond with the cell surface. The longer half-life of covalently bound C4d makes it a footprint of complement activation long after weakly bound antibodies have been cleared by the blood stream. C4 has also been linked to susceptibility to systemic lupus erythematosus and rheumatoid arthritis.

Aucune reactivité croisée: Souris, Boeuf (Vache), Cobaye, Chat, Lapin, Mouton, Chien, Rat (Rattus)

Purification: Protein A purified

Immunogène: Native, from human plasma.

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA (1:5000 - 1:20000), IF (1:250 - 1:600), IHC, IHC-Fr (1:100 - 1:750), IHC-P, WB

Usage: Immunohistochemistry: It has been reported that this antibody works very well on acetone-fixed, frozen renal biopsies. Strong staining is observed in the glomeruli and in some cases the peritubular capillaries. Clone 10-11 has given variable results on formalin-fixed, paraffin-embedded sections. It has been observed that pre-treatment with 88 % formic acid for 20 minutes at room temperature is beneficial (6).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.4, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Short term: -20°C
Long term: -20°C.

Détails sur Complement C4d

Antigène: Complement C4d (C4d) (Complement Component C4d (C4d))

Autre désignation: Complement C4d