VCAM1 anticorps

N° du produit ABIN208158

Cet anticorps anti-VCAM1 est un anticorps Monoclonal produit chez le Souris, détectant VCAM1 dans FACS, IHC, IP et IHC (fro). Convient pour Humain.

Aperçu rapide pour VCAM1 anticorps (ABIN208158)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VCAM1 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-VCAM1 anticorps

Specificité: Reacts with human VCAM-1.

Purification: Purified

Immunogène:

Type of Immunogen: Native protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: Flo (1:10), IHC, IHC-Fr, IP

Usage: Useful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation. Flow Cytometry: 1:10. Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1 % paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/mL), IL-1 (100U/mL) or LPS (1 μg/mL) in tissue culture medium at 37?C. As negative control it is advised to use a control murine IgG1 antibody.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur VCAM1

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: VCAM1 / VCAM-1 / CD106

Sujet: Name/Gene ID: VCAM1
Family: ICAM

Synonyms: VCAM1, CD106, CD106 antigen, INCAM-100, V-CAM 1, VCAM-1

ID gène: 7412

UniProt: P19320

Pathways: Carbohydrate Homeostasis