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SMC1A anticorps (pSer957)

L’anticorps Souris Monoclonal anti-SMC1A a été validé pour WB. Il convient pour détecter SMC1A dans des échantillons de Humain, Boeuf (Vache), Chien, Lapin et Singe.
N° du produit ABIN211403

Aperçu rapide pour SMC1A anticorps (pSer957) (ABIN211403)

Antigène

Voir toutes SMC1A Anticorps
SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivité

  • 131
  • 55
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  • 6
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Humain, Boeuf (Vache), Chien, Lapin, Singe

Hôte

  • 113
  • 13
  • 4
  • 1
Souris

Clonalité

  • 98
  • 33
Monoclonal

Conjugué

  • 68
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  • 4
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  • 1
Cet anticorp SMC1A est non-conjugé

Application

  • 97
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  • 13
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Western Blotting (WB)
  • Épitope

    • 25
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    AA 951-962, pSer957

    Specificité

    Recognizes phospho-SMC1 (Ser957), Mr 150kD. Species cross-reactivity: Human and mouse. Predicted to cross-react with Xenopus and bovine based on sequence homology.

    Purification

    Protein G purified

    Immunogène

    KLH-conjugated, synthetic peptide corresponding to amino acids 951-962 SQEEGS[pS]QGEDS) of human SMC1 (Structural Maintenance of Chromosome 1). The immunizing sequence is identical in bovine and has 11/12 identical amino acids in mouse and xenopus.

    Type of Immunogen: Synthetic peptide

    Isotype

    IgG1
  • Indications d'application

    Approved: WB (1 - 2 μg/mL)

    Usage: Suitable for use in Western Blot. Western Blot: 1-2 μg/mL detects phospho-SMC1 in RIPA lysates from irradiated GMO536 cells and hydroxyurea treated HEK293 cells. Cell lysates from untreated and irradiated GMO536 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-SMC1 (Ser957) (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Commentaires

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Antigène

    SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

    Autre désignation

    SMC1A / SMC1

    Sujet

    Name/Gene ID: SMC1A

    Synonyms: SMC1A, CDLS2, DXS423E, KIAA0178, SB1.8, SMC protein 1A, SMC1, SMC1L1, SMC-1-alpha, SMC-1A, SMCB

    ID gène

    8243

    UniProt

    Q14683

    Pathways

    Stem Cell Maintenance
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