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GAPDH anticorps

Cet anticorps anti-GAPDH est un anticorps Souris Monoclonal détectant GAPDH dans WB, ELISA, IF et ICC. Adapté pour Humain, Souris, Rat, Lapin, Poulet et Grenouille.
N° du produit ABIN211932

Aperçu rapide pour GAPDH anticorps (ABIN211932)

Antigène

Voir toutes GAPDH Anticorps
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité

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Humain, Souris, Rat, Lapin, Poulet, Grenouille

Hôte

  • 168
  • 90
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  • 9
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Souris

Clonalité

  • 186
  • 99
Monoclonal

Conjugué

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Cet anticorp GAPDH est non-conjugé

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Specificité

    Recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit skeletal muscle. Also recognizes cardiac GAPDH. Species cross-reactivity: human, rat, mouse, chicken, frog and fish skeletal muscle. Does not react with GAPDH from E. coli. GAPDH enzyme is also detected in many non-muscle cell lines including HeLa, HCT-116, U937, and THP-1 cells.

    Purification

    Protein G purified

    Immunogène

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from rabbit muscle.

    Type of Immunogen: Purified protein

    Isotype

    IgG1
  • Indications d'application

    Approved: ELISA (1:1000000), ICC (1:100 - 1:300), IF, WB (1:500)

    Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunofluorescence. ELISA: 1:1000000. Western Blot: 1:500. Detects on 10 μg of A431 lysates. Immunocytochemistry: 1:100-1:300. Dilute in PBS, 10 mg/mL BSA. Fixative: 4 % PFA, 0.02 % PBS-Triton X-100. Incubation time: 3 min at RT.

    Commentaires

    Target Species of Antibody: Rabbit

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.4, 0.1 % sodium azide. glycerol-free.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    avoid freezing

    Stock

    4 °C

    Stockage commentaire

    4°C, avoid freezing.
  • Antigène

    GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

    Autre désignation

    GAPDH

    Sujet

    Name/Gene ID: GAPDH

    Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit

    ID gène

    2597

    UniProt

    P04406
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