PLK1 anticorps (pThr210)

N° du produit ABIN214445

L’anticorps anti-PLK1 Polyclonal Lapin est utilisé pour la détection de PLK1 dans des échantillons de Humain. Il a été validé pour WB, ELISA, IHC et IHC (p).

Aperçu rapide pour PLK1 anticorps (pThr210) (ABIN214445)

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PLK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PLK1 anticorps

Épitope: pThr210

Specificité: Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.

Purification: Immunoaffinity purified

Immunogène:

Type of Immunogen: Native protein

Information d'application

Indications d'application: Approved: ELISA (1:3000 - 1:12000), IHC, IHC-P (10 μg/mL), WB (1:200 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.Western Blot: It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur PLK1

Antigène: PLK1 (Polo-Like Kinase 1 (PLK1))

Autre désignation: PLK1 / PLK-1

Sujet: Name/Gene ID: PLK1
Subfamily: Polo
Family: Protein Kinase

Synonyms: PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1

ID gène: 5347

UniProt: P53350

Pathways: Cycle Cellulaire, M Phase