Fc gamma RII (CD32) anticorps (FITC)

N° du produit ABIN2144558

L’anticorps Souris Monoclonal anti- a été validé pour FACS. Il convient pour détecter dans des échantillons de Humain.

Aperçu rapide pour Fc gamma RII (CD32) anticorps (FITC) (ABIN2144558)

Antigène: Fc gamma RII (CD32)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: FITC

Application: Flow Cytometry (FACS)

Clone: 4AIV-3

Détail du produit

Attributs du produit: 4AIV-3 reacts with an low affinity receptor for aggregated IgG (FcgRII), 40 kD. CD32 Molecule is expressed on B cells, monocytes, granulocytes and platelets. This clone also cross-reacts with monocytes, granulocytes and subset of peripheral blood lymphocytes of non-human primates.The reactivity on leukocyte populations is similar to that Obs.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Procédure de l'essai:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32