GLI3 anticorps (AA 41-57)

N° du produit ABIN214604

L’anticorps anti-GLI3 Polyclonal Lapin est utilisé pour la détection de GLI3 dans des échantillons de Humain, Poulet, Chien, Xenopus laevis, Caille et Squirrel Monkey. Il a été validé pour WB, IHC, IF, ELISA, ICC et IHC (p).

Aperçu rapide pour GLI3 anticorps (AA 41-57) (ABIN214604)

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivité: Humain, Poulet, Chien, Xenopus laevis, Caille, Squirrel Monkey

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-GLI3 anticorps

Épitope: AA 41-57

Specificité: Amino acids 41-57 of human Gli-3 protein.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from human GLI3.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA (1:6000 - 1:30000), ICC, IF, IHC, IHC-P (1.25 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1.25 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur GLI3

Antigène: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Autre désignation: GLI3

Sujet: Name/Gene ID: GLI3

Synonyms: GLI3, ACLS, GCPS, GLI-Kruppel family member GLI3, GLI3 full length protein, GLI3-190, GLI family zinc finger 3, Gli-3, GLI3FL, PAP-A, PAPA1, PHS, PPDIV, Transcriptional activator GLI3, PAPA, PAPB, GLI3 form of 190 kDa, Oncogene GLI3, Zinc finger protein GLI3

ID gène: 2737

UniProt: P10071

Pathways: Signalisation Hedgehog