Desmocollin 1 anticorps (Intracellular)

N° du produit ABIN215762

L’anticorps Souris Monoclonal anti-Desmocollin 1 a été validé pour WB, IHC, IHC (p) et IHC (fro). Il convient pour détecter Desmocollin 1 dans des échantillons de Humain.

Aperçu rapide pour Desmocollin 1 anticorps (Intracellular) (ABIN215762)

Antigène: Desmocollin 1 (DSC1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmocollin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Desmocollin 1 anticorps

Épitope: Intracellular

Specificité: Antigen recognized in species specific for human. Cell Lines (tested so far) of differentiated cells. All simple epithelia are negative. desmocollin 1, negative with bovine (tested so far) and rodent material. Reacts with 111kD (form 'a') and 103kD (form 'b') polypeptides (desmocollin1 splice forms, both at pI 5.2) from human epidermal desmosomes. Reactivities on Cultured Positive on primary keratinocytes, however, restricted to local piles.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Monkey (96%) Gorilla (92%) Marmoset (88%).

Purification: Protein A purified

Immunogène: Synthetic peptide corresponding to sequence present within the intracellular part of human desmocollin 1 (HQTLESVKGVGQGDTGRYATDWQC). Percent identity by BLAST analysis: Human, Gibbon, Monkey (96%), Gorilla (92%), Marmoset (88%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Immunohistochemistry and Western Blot. Immunohistochemistry (Frozen, Paraffin, HEIR): 1:10 in PBS,  pH 7.4, Incubation Time 1 hr at RT or overnight at 4?C. Note: For better resolution on frozen sections preincubation (directly after fixation) with 0.05-0.2 % Triton X-100, for 5-10 min, depending on tissue type, is recommended (see ref. 2). Without detergent pretreatment a cytoplasmic component might cross-react (e. g. in fibroblasts). (Paraffin: slight cross-reactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step. Localization: Localizes Dsc1 present in suprabasal layers of interfollicular epidermis, specific cell layers (e. g. in hair follicle root sheath), Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with 1 mL sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized powder from PBS,  pH 7.4, 0.5 % BSA, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Détails sur Desmocollin 1

Antigène: Desmocollin 1 (DSC1)

Autre désignation: DSC1 / Desmocollin 1

Sujet: Name/Gene ID: DSC1
Family: Cadherin

Synonyms: DSC1, Desmocollin-1, Desmocollin 1, Desmosomal glycoprotein 2/3, DG2/DG3, Cadherin family member 1, CDHF1

ID gène: 1823

UniProt: Q08554