IL-5 anticorps (Biotin)

N° du produit ABIN218119

Cet anticorps Rat Monoclonal détecte spécifiquement IL-5 dans WB, ELISA, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour IL-5 anticorps (Biotin) (ABIN218119)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Humain

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-5 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-IL-5 anticorps (Biotin)

Specificité: Recognizes human Interleukin-5 (IL-5).

Purification: Purified

Immunogène: COS-expressed recombinant human IL-5.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: ELISA, Flo, IF, WB

Usage: ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blotting applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-5 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur IL-5

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: IL5

Sujet: Name/Gene ID: IL5
Family: Hormone

Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

ID gène: 3567

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour