IL-5 anticorps

N° du produit ABIN218123

L’anticorps Rat Monoclonal anti-IL-5 a été validé pour WB, ELISA, FACS, Neut et IF. Il convient pour détecter IL-5 dans des échantillons de Humain et Souris.

Aperçu rapide pour IL-5 anticorps (ABIN218123)

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-5 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut), Immunofluorescence (IF)

Détail du produit anti-IL-5 anticorps

Specificité: Recognizes mouse and human Interleukin-5 (IL-5).

Purification: Purified

Immunogène: Semi-purified mouse T cell clone supernatant.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, Flo, IF, Neut, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels.5-7 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.8 Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-5 under reducing conditions. Neutralization: The LE/AF form can be used for neutralizing mouse IL-5 bioactivity. A suitable LE/AF Rat IgG1 should be used as an isotype control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS. No preservative added.

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Détails sur IL-5

Antigène: IL-5 (IL5) (Interleukin 5 (IL5))

Autre désignation: IL5

Sujet: Name/Gene ID: IL5
Family: Hormone

Synonyms: IL5, EDF, Interleukin-5, IL-5, T-cell replacing factor, TRF

ID gène: 3567

UniProt: P05113

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour