TLR9 anticorps

N° du produit ABIN2191784

L’anticorps Souris Monoclonal anti-TLR9 a été validé pour WB, FACS, IHC (p), IHC (fro), IA et D. Il convient pour détecter TLR9 dans des échantillons de Souris. Il y a 2+ publications disponibles.

Aperçu rapide pour TLR9 anticorps (ABIN2191784)

Antigène: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TLR9 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA), Detection (D)

Clone: 5G5

Détail du produit anti-TLR9 anticorps

Stérilité: 0.2 μm filtered

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-TLR9 (ABIN2191784)

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." dans: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, (2008) (PubMed).

Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." dans: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, (2004) (PubMed).

Détails sur TLR9

Antigène: TLR9 (Toll-Like Receptor 9 (TLR9))

Autre désignation: Toll-Like Receptor 9

Sujet: The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG- dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades