Polymyxin B anticorps

N° du produit ABIN2191915

Détail du produit anti-Polymyxin B anticorps

Antigène: Polymyxin B

Reactivité: Chemical

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Polymyxin B est non-conjugé

Application: Western Blotting (WB)

niveau d'endotoxine: Low endotoxin level

Clone: 45

Information d'application

Indications d'application: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Stockage

Buffer: 1 mL(> 200 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20 °C Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-Polymyxin B (ABIN2191915)

Appelmelk, Su, Verweij-van Vught, Thijs, MacLaren: "Polymyxin B-horseradish peroxidase conjugates as tools in endotoxin research." dans: Analytical biochemistry, Vol. 207, Issue 2, pp. 311-6, (1993) (PubMed).

Détails sur Polymyxin B

Antigène: Polymyxin B

Abstract: Polymyxin B Produits

Classe de substances: Chemical

Sujet: The monoclonal antibody 45 reacts with Polymyxin B. The antibody binds to free Polymyxin B as well as to Polymyxin B already bound to LPS. The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody 45 enables the possibilities to study quantitatively the interaction of PMB and LPS.