CKB anticorps

N° du produit ABIN2191988

Détail du produit anti-CKB anticorps

Antigène: CKB (Creatine Kinase, Brain (CKB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CKB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Réactivité croisée (Details): Cross reactivity: Rabbit : Yes, Mouse : Yes, Rat : Yes

Stérilité: 0.2 μm filtered

Clone: CK-BYK-21E10

Isotype: IgG2b

Information d'application

Indications d'application: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human Purkinje cells control Negative Human fibroblasts control

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Références pour anticorps anti-CKB (ABIN2191988)

Sistermans, de Kok, Peters, Ginsel, Jap, Wieringa: "Tissue- and cell-specific distribution of creatine kinase B: a new and highly specific monoclonal antibody for use in immunohistochemistry." dans: Cell and tissue research, Vol. 280, Issue 2, pp. 435-46, (1995) (PubMed).

de Kok, Geurds, Sistermans, Usmany, Vlak, Wieringa: "Production of native creatine kinase B in insect cells using a baculovirus expression vector." dans: Molecular and cellular biochemistry, Vol. 143, Issue 1, pp. 59-65, (1995) (PubMed).

Détails sur CKB

Antigène: CKB (Creatine Kinase, Brain (CKB))

Autre désignation: Creatine Kinase B-Type (CKB Produits)

Synonymes: anticorps B-CK, anticorps CKBB, anticorps b-ck, anticorps ckbb, anticorps CKB, anticorps Bck, anticorps Ck-3, anticorps Ck3, anticorps Ckbb, anticorps bck, anticorps ckb, anticorps cb594, anticorps wu:fj36f10, anticorps wu:fj37d11, anticorps Ckbr, anticorps mg:cb02h12, anticorps wu:fb62d04, anticorps wu:fc14g11, anticorps zgc:154095, anticorps zgc:92079, anticorps creatine kinase B, anticorps creatine kinase, brain L homeolog, anticorps creatine kinase B-type, anticorps creatine kinase, brain, anticorps creatine kinase, brain b, anticorps creatine kinase, brain a, anticorps CKB, anticorps ckb.L, anticorps Ckb, anticorps ckb, anticorps ckbb, anticorps ckba

Sujet: The monoclonal antibody CK-BYK/21E10 recognizes human creatine kinase B-type, also known as CKB. Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development. Aliases Creatine kinase B chain, B-CK, BB-CK Immunogen Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain- type (SNSHNALKLRFPAEDEF)