Galectin 3 anticorps

N° du produit ABIN2192045

Détail du produit anti-Galectin 3 anticorps

Antigène: Galectin 3 (LGALS3)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Galectin 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoassay (IA)

Stérilité: 0.2 μm filtered

Clone: B2C10

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-Galectin 3 (ABIN2192045)

Feilchenfeldt, Tötsch, Sheu, Robert, Spiliopoulos, Frilling, Schmid, Meier: "Expression of galectin-3 in normal and malignant thyroid tissue by quantitative PCR and immunohistochemistry." dans: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 16, Issue 11, pp. 1117-23, (2003) (PubMed).

Sano, Hsu, Yu, Apgar, Kuwabara, Yamanaka, Hirashima, Liu: "Human galectin-3 is a novel chemoattractant for monocytes and macrophages." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2156-64, (2000) (PubMed).

Détails sur Galectin 3

Antigène: Galectin 3 (LGALS3)

Autre désignation: Galectin-3 (LGALS3 Produits)

Synonymes: anticorps CBP 35, anticorps Gal-3, anticorps Galectin-3, anticorps leg3, anticorps galactoside-binding, anticorps galectin-3, anticorps lectin, anticorps soluble, anticorps CBP35, anticorps GAL3, anticorps GALBP, anticorps GALIG, anticorps L31, anticorps LGALS2, anticorps MAC2, anticorps MGC52947, anticorps MGC75803, anticorps LGALS3, anticorps gal-3, anticorps GBP, anticorps L-34, anticorps Mac-2, anticorps gal3, anticorps galectin 3, anticorps Galectin-3, anticorps avian beta-defensin 3, anticorps lectin, galactoside-binding, soluble, 3 L homeolog, anticorps lectin, galactoside-binding, soluble, 3, anticorps galectin 2, anticorps lectin, galactose binding, soluble 3, anticorps LGALS3, anticorps leg3, anticorps AvBD3, anticorps lgals3.L, anticorps lgals3, anticorps LGALS2, anticorps Lgals3

Classe de substances: Chemical

Sujet: The monoclonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain, a carboxyl- terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up- regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions, galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP), a factor in pre-mRNA splicing, and has been found to control cell cycle and prevent T cell apoptosis. On the other hand, this protein has also been demonstrated to function as extracellular molecule in activating various types of cells, including monocytes/macrophages, mast cells, neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates, the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.

Pathways: Signalisation RTK