Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CD55 anticorps

CD55 Reactivité: Rat WB, Func Hôte: Souris Monoclonal RDIII-7 unconjugated
N° du produit ABIN2192123
  • Antigène Voir toutes CD55 Anticorps
    CD55 (Complement Decay-Accelerating Factor (CD55))
    Reactivité
    • 117
    • 36
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    Rat
    Hôte
    • 73
    • 69
    • 6
    • 6
    • 4
    • 1
    • 1
    Souris
    Clonalité
    • 89
    • 69
    • 2
    Monoclonal
    Conjugué
    • 74
    • 18
    • 16
    • 12
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CD55 est non-conjugé
    Application
    • 97
    • 83
    • 47
    • 39
    • 33
    • 26
    • 16
    • 15
    • 14
    • 13
    • 9
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Functional Studies (Func)
    Stérilité
    0.2 μm filtered
    Clone
    RDIII-7
    Top Product
    Discover our top product CD55 Anticorps primaire
  • Indications d'application
    For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.1 % bovine serum albumin.
    Stock
    4 °C
    Stockage commentaire
    Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
    Date de péremption
    12 months
  • Mizuno, Harris, Morgan: "Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury." dans: Journal of reproductive immunology, Vol. 69, Issue 1, pp. 23-34, (2006) (PubMed).

    Mizuno, Donev, Harris, Morgan: "CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa." dans: Molecular immunology, Vol. 44, Issue 7, pp. 1613-22, (2006) (PubMed).

    Harris, Williams, Linton, Morgan: "Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo." dans: Clinical and experimental immunology, Vol. 129, Issue 2, pp. 198-207, (2002) (PubMed).

    Harris, Spiller, Morgan: "Human and rodent decay-accelerating factors (CD55) are not species restricted in their complement-inhibiting activities." dans: Immunology, Vol. 100, Issue 4, pp. 462-70, (2000) (PubMed).

    Spiller, Harris, Morgan: "Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells." dans: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 51-60, (1999) (PubMed).

  • Antigène
    CD55 (Complement Decay-Accelerating Factor (CD55))
    Autre désignation
    Complement Decay Accelerating Factor (CD55 Produits)
    Synonymes
    anticorps CR, anticorps CROM, anticorps DAF, anticorps TC, anticorps Daf, anticorps Daf-GPI, anticorps Daf1, anticorps GPI-DAF, anticorps CD55 molecule (Cromer blood group), anticorps CD55 molecule, decay accelerating factor for complement, anticorps CD55, anticorps Cd55
    Sujet
    The monoclonal antibody RDIII-7 recognizes complement decay accelerating factor (DAF), also designated as CD55. RDIII-7 recognizes the common extracellular SCR region of CD55, detecting all isoforms of the protein. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half- life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Aliases DAF, CD55 Immunogen NIH-3T3 cells expressing GPI-anchored rat DAF
    Pathways
    Système du Complément, Regulation of Leukocyte Mediated Immunity
Vous êtes ici:
Support technique