CD59 anticorps

N° du produit ABIN2192125

Détail du produit anti-CD59 anticorps

Antigène: CD59

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD59 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func)

Stérilité: 0.2 μm filtered

Clone: TH9

Information d'application

Indications d'application: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.

Restrictions: For Research Use only

Stockage

Buffer: PBS, containing 0.1 % bovine serum albumin.

Stock: 4 °C

Stockage commentaire: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Date de péremption: 12 months

Références pour anticorps anti-CD59 (ABIN2192125)

Rogers, Gasque, Piddlesden, Okada, Holers, Morgan: "Expression and function of membrane regulators of complement on rat astrocytes in culture." dans: Immunology, Vol. 88, Issue 1, pp. 153-61, (1996) (PubMed).

Hughes, Meri, Davies, Williams, Morgan: "Immunolocalization and characterization of the rat analogue of human CD59 in kidney and glomerular cells." dans: Immunology, Vol. 80, Issue 3, pp. 439-44, (1994) (PubMed).

Hughes, Piddlesden, Williams, Harrison, Morgan: "Isolation and characterization of a membrane protein from rat erythrocytes which inhibits lysis by the membrane attack complex of rat complement." dans: The Biochemical journal, Vol. 284 ( Pt 1), pp. 169-76, (1992) (PubMed).

Détails sur CD59

Antigène: CD59

Autre désignation: CD59 Glycoprotein (CD59 Produits)

Synonymes: anticorps 16.3A5, anticorps 1F5, anticorps EJ16, anticorps EJ30, anticorps EL32, anticorps G344, anticorps HRF-20, anticorps HRF20, anticorps MAC-IP, anticorps MACIF, anticorps MEM43, anticorps MIC11, anticorps MIN1, anticorps MIN2, anticorps MIN3, anticorps MIRL, anticorps MSK21, anticorps p18-20, anticorps Cd59a, anticorps Cd59b, anticorps MACIP, anticorps CD59 molecule (CD59 blood group), anticorps CD59 molecule, anticorps CD59, anticorps Cd59

Sujet: The monoclonal antibody TH9 recognizes rat CD59. Rat CD59 was previously known as rat inhibitory protein (RIP) and it is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 regulates the formation and function of the lytic C5b-9 complex by binding C8 and preventing the unfolding and membrane insertion of C9 and by binding C9 and restricting its polymerization.CD59 is a small (18 - 25 kDa) molecule, linked to the cell membrane through a glycosyl phosphatidylinositol (GPI) anchor and comprising 77 amino acids with a single N-linked carbohydrate group at Asn-18. Analogues of CD59 can be found in all species with similar structures and sizes. In rat, CD59 is expressed on vacular endothelium and circulating cells. In the central nervous system (CNS) of the rat, CD59 is expressed on the Schwann sheath of peripheral nerve fibres and on ependymal cells, but not on glial cells and neurons in the CNS. Rat astrocytes in vitro express CD59 on its surface. CD59 may be involved in rheumatoid arthritis, motor nerve injury in the Guillain-Barré sydrome and in other diseases where defective inhibition of complement activation on self tissue is involved. Furthermore, CD59 may play an important part in abrogating the effects of complement attack in renal disease. Its presence and protective effect have already been demonstrated on human renal cells. Aliases Membrane attack complex inhibition factor, MACIF, MAC-inhibitory protein, Protectin, CD59 Immunogen Partially purified (FPLC) rat CD59 prep (RIP)

Pathways: Système du Complément