Choline Acetyltransferase anticorps

N° du produit ABIN224453

L’anticorps Souris Monoclonal anti-Choline Acetyltransferase a été validé pour WB, IHC, ELISA et IHC (pfa). Il convient pour détecter Choline Acetyltransferase dans des échantillons de Humain, Rat et Porc.

Aperçu rapide pour Choline Acetyltransferase anticorps (ABIN224453)

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivité: Humain, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Choline Acetyltransferase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraformaldehyde) (IHC (pfa))

Clone: 1-B3-9B3

Détail du produit anti-Choline Acetyltransferase anticorps

Specificité: Recognizes choline acetyltransferase (ChAT)-positive cells. Species cross-reactivity: Human, Rat and Porcine.

Purification: Purified

Immunogène: Highly purified ChAT obtained from porcine brain

Isotype: IgG1

Information d'application

Indications d'application: Approved: ELISA, IHC, IHC-Pfa (10 - 20 μg/mL), WB

Usage: Fixation: a) Human tissue: Fix by placing fresh (5-8 h post mortem) tissue in a 1:1 solution of 4 % paraformaldehyde/10 % formaldehyde for 1 week at 4 °C. Divide into tissue blocks (basal forebrain, septum and basal ganglia). Incubate the blocks in sucrose solutions of increasing concentration (10 %, 20 % and 30 %). Prepare 50um sections and store in a cryoprotective solution at -15 °C (3).b) Rat tissue: Brain and spinal cord marrow tissue (transcardial perfusion). Perform a transcardial perfusion initially with oxygen-saturated heparin solution (II) (100-200ml) and subsequently with paraformaldehyde/picric acid solution (III) (400ml). Carry out immediate tissue preparation and fix for a further 30 minutes in the same solution. Wash out the fixative with several fresh quantities of Buffer A. Prepare 50um sections.

Commentaires:

Target Species of Antibody: Pig

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: avoid freeze thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Détails sur Choline Acetyltransferase

Antigène: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Autre désignation: CHAT

Sujet: Name/Gene ID: CHAT

Synonyms: CHAT, Choline acetylase, Choline acetyltransferase, Choline O-acetyltransferase, CMS1A2, CHOACTASE, CMS1A

ID gène: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development