SMAD3 anticorps (Internal Region)

N° du produit ABIN233815

N° du produit (Fournisseur): 600-401-973

Cet anticorps Lapin Polyclonal détecte spécifiquement SMAD3 dans WB et ELISA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour SMAD3 anticorps (Internal Region) (ABIN233815)

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD3 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-SMAD3 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-973

Fournisseur: Rockland

Fonction: SMAD3 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human Smad3 protein.

Attributs du produit: Synonyms: rabbit anti-SMAD3 antibody, SMAD-3, SMAD 3, mothers against decapentaplegic homolog 3 antibody, MAD homolog 3, Mothers against DPP homolog 3, SMAD family member 3, MADH3, MADH 3, JV15-2

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 50 kDa in size corresponding to Smad3 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:10,000

ELISA Dilution: 1:15,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur SMAD3

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: SMAD3

Sujet: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-b (transforming growth factor) and activin type I receptor kinase.  These activators exert diverse effects on a wide array of cellular processes.  The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway.  The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-b-mediated responses.

ID gène: 4088, 5174513

UniProt: P84022

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy