HLAG anticorps (C-Term)

N° du produit ABIN238372

Cet anticorps Souris Monoclonal détecte spécifiquement HLAG dans FACS, WB, IHC (fro), IHC (p), IF, EIA et ELISA (Capture). Il présente une réactivité envers Humain.

Aperçu rapide pour HLAG anticorps (C-Term) (ABIN238372)

Antigène: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HLAG est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: 5A6G7

Détail du produit anti-HLAG anticorps

Épitope: C-Term

Specificité: The antibody 5A6G7 allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.

Réactivité croisée (Details): Species reactivity (tested):Human

Attributs du produit: Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen

Purification: Protein A chromatography

Immunogène: C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupledto ovalbumin.

Isotype: IgG1

Information d'application

Indications d'application: Western Blotting (Reducing Conditions): 12 % AA SDS-PAGE. Positive Control: JEG-3 cell lysate. Flow Cytometry. Immunohistochemistry on Paraffin and Frozen Sections: 10-20 μg/mL. Positive tissue: placenta tissue. Immunocytochemistry. ELISA: The antibody 5A6G7 has been tested as the capture antibody in a sandwich ELISAfor analysis of soluble HLA-G in combination with antibody ABIN238525. Positive Control: HeLa/HLA-G5 transfectants cell lysate. HeLa/HLA-G5 cell supernatant. Negative Control: HeLa cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 with 15 mM Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur HLAG

Antigène: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Autre désignation: HLA Class I alpha G / HLA-G

Sujet: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G

ID gène: 3135

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response