CK1 epsilon anticorps (C-Term)

N° du produit ABIN238540

Cet anticorps Chèvre Polyclonal détecte spécifiquement CK1 epsilon dans WB, ELISA, IHC, IF et FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour CK1 epsilon anticorps (C-Term) (ABIN238540)

Antigène: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CK1 epsilon est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-CK1 epsilon anticorps

Épitope: C-Term

Fonction: CSNK1E

Séquence: PASQTSVPFD HLGK

Réactivité croisée: Chien, Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PASQTSVPFDHLGK, from the C Terminus of the protein sequence according to NP_001885.1, NP_689407.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5 μg/mL.

Western Blot: Approx 48 kDa band observed in nuclear lysates of cell lines A431and HeLa, and approx 45 kDa in nuclear lysates of cell line K562 (calculated MW of 47.3 kDa according to NP_001276841.1). Recommended concentration: 0.3-1 μg/mL. Primary incubatio

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur CK1 epsilon

Antigène: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Autre désignation: CSNK1E

Sujet: CSNK1E, casein kinase 1, epsilon, RP1-5O6.1, HCKIE, MGC10398 , CKI-epsilon, OTTHUMP00000063258, OTTHUMP00000063263, casein kinase 1 epsilon, casein kinase I epsilon

ID gène: 1454, 27373, 58822

NCBI Accession: NP_001276841

Pathways: Signalisation Hedgehog, M Phase