His Tag anticorps (C-Term, N-Term)

N° du produit ABIN2443717

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, ELISA, IP, IHC et DB. Il a été cité dans 1 publication.

Aperçu rapide pour His Tag anticorps (C-Term, N-Term) (ABIN2443717)

Antigène: His Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)

Clone: HIS-H8

Détail du produit anti-His Tag anticorps

Épitope: C-Term, N-Term

Fonction: His Tag Mouse Monoclonal Antibody (HIS.H8)

Séquence: HHHHHH

Specificité: The antibody recognizes His-tag encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags)

Purification: Protein G purified

Immunogène: The antibody was raised against HHHHHH (6x His) synthetic peptide conjugated to KLH.

Isotype: IgG2b

Information d'application

Indications d'application: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Références pour anticorps anti-His Tag (ABIN2443717)

Bouvette, Korkut, Fouillen, Amellah, Nanci, Durocher, Omichinski, Legault: "High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension." dans: BMC biotechnology, Vol. 18, Issue 1, pp. 76, (2019) (PubMed).

Détails sur His Tag

Antigène: His Tag

Classe de substances: Tag

Sujet: Epitope tags have applications in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size, the protein's biochemical properties seem not to be affected by the tag protein. Polyhistidine-tag is also known as histidine-tag, 6xHis-tag and by the trademarked name His-tag.