DYKDDDDK Tag anticorps (C-Term, N-Term)

N° du produit ABIN2443789

Cet anticorps Souris Monoclonal détecte spécifiquement DYKDDDDK Tag dans WB, ELISA, IP, IHC et DB.

Aperçu rapide pour DYKDDDDK Tag anticorps (C-Term, N-Term) (ABIN2443789)

Antigène: DYKDDDDK Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DYKDDDDK Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Dot Blot (DB)

Clone: FG4R

Détail du produit anti-DYKDDDDK Tag anticorps

Épitope: C-Term, N-Term

Fonction: FLAG Tag Mouse Monoclonal Antibody (FG4R)

Séquence: DYKDDDDK

Specificité: The antibody recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins.

Purification: Protein G purified

Immunogène: The antibody was raised against DYKDDDDK (FLAG) synthetic peptide conjugated to KLH.

Isotype: IgG2b

Information d'application

Indications d'application: WB 1:1000,IHC 1:500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB 1:1000-3,000, IHC 1:500-2,000. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur DYKDDDDK Tag

Antigène: DYKDDDDK Tag

Autre désignation: FLAG Tag

Classe de substances: Tag

Sujet: Epitope tags have application in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein's biochemical properties seem not to be affected by the tag protein. FLAG-tag, or FLAG octapeptide, a polypeptide consisting of eight amino acids (DYKDDDDK) can be added to a protein using recombinant DNA technology, therefore, the tag can be detected with the fused protein in many different assays that require recognition by an antibody.