ITK anticorps (AA 1-26)

N° du produit ABIN2443927

Cet anticorps Souris Monoclonal détecte spécifiquement ITK dans WB, IP et EAA. Il présente une réactivité avec des échantillons de Humain et Rat.

Aperçu rapide pour ITK anticorps (AA 1-26) (ABIN2443927)

Antigène: ITK (IL2-Inducible T-Cell Kinase (ITK))

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITK est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Activity Assay (EAA)

Clone: 2F12

Détail du produit anti-ITK anticorps

Épitope: AA 1-26

Fonction: Itk (IL2-inducible T-cell kinase) Mouse Monoclonal Antibody (2F12)

Specificité: A highly specific and sensitive antibody against Itk. The antibody recognizes both native and denatured Itk (amino acids 1-26). No detectable cross reactivity with other Itk family members such as Btk or Tec. S

Purification: Protein G purified

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100μL: WB 2 μg/mL. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Date de péremption: 24 months

Détails sur ITK

Antigène: ITK (IL2-Inducible T-Cell Kinase (ITK))

Autre désignation: Itk (IL2-inducible T-cell kinase)

Sujet: IL2-inducible T-cell kinase (Itk) is located in cell membrane of T-cell lines and natural killer cell lines and plays a role in T-cell proliferation and differentiation. Defects in Itk are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1).

UniProt: Q08881

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway